human ifnγ elisa kit Search Results


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ifn  (Cusabio)
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Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
Human Ifn Gamma Elisa Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
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Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
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Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
Human Ifn Gamma Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human ifn gamma quantikine kit
Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
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Assaypro assaymax human interferon gamma ifn γ elisa kit
Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by <t>ELISA</t> after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f
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Image Search Results


Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by ELISA after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Immunological synapse formation as a key mechanism in T cell-dependent bispecific antibody-mediated immune activation and cytotoxicity

doi: 10.1007/s00262-025-04036-w

Figure Lengend Snippet: Cytotoxic activity and inflammatory cytokine release in co-culture systems. a–c After co-culturing WT or KO, T cells, and TDB, the color reaction with WST-8 reagent in an hour was measured. The co-culture times were 24 h, 48 h, and 72 h of co-culture, respectively. Red: WT + T cell + TDB, blue: KO + T cell + TDB. Error bars represent the mean ± SD ( n = 6), ** p < 0.005, *** p < 0.0005, n.s. not significant. d Measurement of IFN-γ release by ELISA after 24 h of co-culture time. Co-culture supernatants were obtained and used for assay using the ELISA Kit. Red: WT + T cell + TDB, blue: KO + T cell + TDB, gray: negative control (T cell + TDB). Red letters are the results of the WT and N/C statistical tests, and blue letters are the results of the KO and N/C statistical tests. Error bars represent the mean ± SD ( n = 3), *** p < 0.0005, n.s. not significant. e IFN-γ release at TDB: 10 ng/mL for each co-culture time. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. f Cancer cell death when the IFN-γ release measured in e is added to the standard. From left to right, IFN-γ release at 6 h, 24 h, 48 h, and 72 h was added. Error bars represent the mean ± SD ( n ≧ 3), *** p < 0.0005, n.s. not significant. g-i TNF-α version of the same experiment as d – f

Article Snippet: T cells (E:T = 5:1, 1.0 × 10 6 cells/50 μL/well) were added and co-cultured. hEx3 was added at a final concentration of 0.1–100 ng/mL in 100 μL of medium and cultured for 24 h. The culture supernatant was then collected and assayed using human IFN-gamma ELISA Kit, human TNF-alpha ELISA Kit, human CXCL10 ELISA Kit (Proteintech, Rosemont, IL), and human HMGB1 ELISA Kit (Chondrex, Woodinville, WA) according to the protocols provided.

Techniques: Activity Assay, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Negative Control